Glutathione (GSH) Colorimetric Detection BioAssay™ Kit

Whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples

Glutathione and Oxidized Glutathione

Absorbance Plate Reader

Quantitatively measure glutathione (GSH) and oxidized glutathione (GSSG) present in a variety of samples, including whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples. Analyze the absorbance colorimetric signal by spectrophotometer.

Glutathione, gamma-L-glutamyl-L-cysteinyl-glycine or GSH, is the most abundant non-protein thiol in cells. GSH is involved in the regulation of a number of essential biochemical processes within the cell. Primarily recognized as a key intracellular source of reducing power for combating the toxic accumulation of free radical byproducts, GSH is also involved with detoxifying and removal of exo/endogenous toxins and alkylating agents. In its role as a cell signaling agent, GSH is involved in DNA synthesis and cell proliferation regulation. Due to the genetically conserved molecular processes by which cells die, intracellular levels of GSH can favor a cell death pathway via apoptosis (adequate intracellular GSH stores) or via necrosis or autophagy (depleted intracellular GSH stores). Detection of a drop in intracellular GSH concentration in an experimental cell population relative to a negative (healthy cell) control is often indicative of an apoptosis induction event. Glutathione Colorimetric Detection BioAssay™ Kit is designed to quantitatively measure GSH and GSSG in a variety of samples.

4 clear half-area polystyrene 96-well microtiter plates
Oxidized Glutathione (GSSG) Standard, 1x250uM, 1x350uL
Colorimetric GSH Detection Substrate, 1x1mL
Glutathione Assay Buffer, 1x225mL
NADPH Concentrate, 1x1mL
Glutathione Reductase Concentrate, 1x1mL

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards