HRV-3C Protease, GST Tag (1U/μL) (active enzyme)

Referencia 3CC-N3133-5000U

embalaje : 5000U

Marca : ACROBiosystems

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  • Product Details
    HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue.
  • Application

    Removal of purification tags from fusion proteins.

  • Unit Definition
    One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h.
  • Purity

    >95% as determined by SDS-PAGE.

  • Enzyme Activity
    >1 U/μL
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Storage

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state.
    2. -70°C for 12 months under sterile conditions after reconstitution.
  • ACRO Quality Management System
    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process
  • SDS-PAGE
    3C (HRV) SDS-PAGE

    The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

    Bioactivity
     3C (HRV) ENZYME

    >1 Units/µg. One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h (QC tested).

    • Clinical and Translational Updates