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> Human CA19-9 ELISA Kit

Human CA19-9 ELISA Kit

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Referencia ELH-CA19-9-1

embalaje : 1PlateKit

Marca : RayBiotech


Human CA19-9 ELISA KitHuman CA19-9 ELISA KitHuman CA19-9 ELISA KitHuman CA19-9 ELISA KitHuman CA19-9 ELISA KitHuman CA19-9 ELISA Kit5 Plate Kit Packaging (one box)

Human CA19-9 ELISA Kit

Human CA19-9 ELISA Kit

RayBio® Human CA19-9 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
0.3 U/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
0.3 U/ml - 200 U/ml
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP- 2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
2 fold
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Shipping TypeBlue ice
Storage≤-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human CA19-9 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified CA19-9, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-CA19-9.pdf)
SDS (ELH-CA19-9.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Impaired sleep efficiency predicts adverse prognosis in elderly pancreatic cancer patients: a retrospective case - control study based on wearable smart devices

    Species:
    Human
    Sample Type:
    Serum (elderly pancreatic cancer patients)
    Year:
    2025
    Journal Name:
    American Journal of Translational Research
    PubMed ID:
    40821080

  • Urinary Carbohydrate Antigen 19-9 in Children with Unilateral Ureteropelvic Junction Obstruction: Does Bladder Urine Reflect Changes in Renal Pelvis?

    Species:
    Human
    Sample Type:
    Urine (urine renal pelvis and bladder)
    Year:
    2025
    Journal Name:
    Journal of Indian Association of Pediatric Surgeons
    PubMed ID:
    41293501

  • GDF15 and LCN2 for early detection and prognosis of pancreatic cancer

    Species:
    Human
    Sample Type:
    Serum (pancreatic cancer patients)
    Year:
    2024
    Journal Name:
    Translational Oncology
    PubMed ID:
    39353236

  • A combination of urinary biomarker panel and PancRISK score for earlier detection of pancreatic cancer: A case-control study

    Species:
    Human
    Sample Type:
    Urine
    Year:
    2020
    Journal Name:
    Plos Medicine
    PubMed ID:
    33301466

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