Human IgG (H+L) Antibody : PE

Referencia OASB02100

embalaje : 0.5mg

Marca : Aviva Systems Biology


Rabbit Anti-Human IgG(H+L) Antibody - PE Conjugated (OASB02100)

Datasheets/ManualsPrintable datasheet for Rabbit Anti-Human IgG(H+L) Antibody - PE Conjugated (OASB02100)
Product Info
Tested Species ReactivityHuman
Predicted Species ReactivityHuman
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ConjugationR-PE
ApplicationFLISA, FC
Additional InformationDescription: Pooled antisera from rabbits hyperimmunized with human IgG; purified by affinity chromatography on human IgG covalently linked to agarose; reacts with the heavy and light chains of human IgG and the light chains of human IgM and IgA
Reconstitution and StorageStore at 2-8C
ImmunogenHuman IgG
PurificationAffinity chromatography on human IgG covalently linked to agarose.
Concentration0.5 mg/mL
SpecificityIgG(H+L)
CharacterizationTo ensure lot- to- lot consistency, each batch of product is tested by ELISA and/or Fluorescent ELISA forconformance to characteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionImmunofluorescence
ELISA:
FITC conjugate
BIOT conjugate
PE conjugate
AP conjugate
HRP conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
Application InfoImmunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry
Other Applications DataSince applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross AbsorptionNA
Gene Full NameIg gamma-1 chain C region