RayBio® Human MMP-3 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human MMP-3 ELISA Kit Standard Curve
Human MMP-3 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 300 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.3 ng/ml - 100 ng/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -7, -8, -9, -10, -12, -13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 - 10 fold |
| Accession Number | P08254 |
| Gene Id | 4314 |
| Gene Symbols | MMP3, STMY1 |
| Protein Name / Synonyms | Stromelysin-1 (SL-1) (EC 3.4.24.17) (Matrix metalloproteinase-3) (MMP-3) (Transin-1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human MMP-3 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified MMP-3 (MMP3), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Electroacupuncture Ameliorates Knee Osteoarthritis By Rebalancing T Cell Homeostasis as Revealed By Immune Repertoire (IR) Sequencing
- Species:
- Human
- Sample Type:
- Serum (KOA patients, baseline and post-treatment)
- Year:
- 2025
- Journal Name:
- Combinatorial chemistry & high throughput screening
- PubMed ID:
- 39136509
IL-40 is up-regulated in the synovial fluid and cartilage of osteoarthritis patients and contributes to the alteration of chondrocytes phenotype in vitro
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (NHAC-kn chondrocytes + IL-40)
- Year:
- 2024
- Journal Name:
- Arthritis Research & Therapy
- PubMed ID:
- 39080724
Chemically modified small interfering RNA targeting Hedgehog signaling pathway for rheumatoid arthritis therapy
- Species:
- Human
- Sample Type:
- fibroblast-like synoviocytes (FLSs)
- Year:
- 2022
- Journal Name:
- Molecular Therapy-Nucleic Acids
Coordination behavior of cocaine toward d-block metal ions: Synthesis, spectral analysis, density functional theory (DFT) studies, and chemotherapeutic activity
- Species:
- Human
- Sample Type:
- MCF-7 cell
- Year:
- 2023
- Journal Name:
- Journal of Molecular Structure
Tonsil-derived mesenchymal stem cells enhance allogeneic bone marrow engraftment via collagen IV degradation
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (MSC-conditioned medium MSC-CM)
- Year:
- 2021
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 34090520
Sanshool improves UVB-induced skin photodamage by targeting JAK2/STAT3-dependent autophagy
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture supernatants of HDFs)
- Year:
- 2019
- Journal Name:
- Cell Death & Disease
- PubMed ID:
- 30622245
The FoxP1 gene regulates lung function, production of matrix metalloproteinases and inflammatory mediators, and viability of lung epithelia
- Species:
- Human
- Sample Type:
- BEAS-2B cell
- Year:
- 2022
- Journal Name:
- Respiratory Research
Sorafenib improves alkylating therapy by blocking induced inflammation, invasion and angiogenesis in breast cancer cells
- Species:
- Human
- Sample Type:
- Conditioned Media (Breast cancer cells)
- Year:
- 2018
- Journal Name:
- Cancer Letters
Inhibition of Inducible Nitric Oxide Synthase Prevents IL-1β-Induced Mitochondrial Dysfunction in Human Chondrocytes
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (IL-1β-stimulated chondrocytes)
- Year:
- 2021
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 33804447
Thioredoxin-1 and Correlations of the Plasma Cytokines Regarding Aortic Valve Stenosis Severity
- Species:
- Human
- Sample Type:
- Plasma (aortic valve stenosis patients)
- Year:
- 2021
- Journal Name:
- Biomedicines
- PubMed ID:
- 34440245