Orb780220-48Tests | Human Myxovirus Resistance 1 (MX1) ELISA Kit Clinisciences

Human Myxovirus Resistance 1 (MX1) ELISA Kit

SKU: orb780220

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myxovirus Resistance 1(MX1).

Research Area

Infectious Disease & Virology

Images & Validation

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Tested Applications	ELISA
Application Notes	standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MX1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MX1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.63 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IFI-78K ELISA Kit, IFI78 ELISA Kit, MX ELISA Kit, MxA ELISA Kit, Interferon-Inducible Protein p78 ELISA Kit, Interferon-regulated resistance GTP-binding protein MxA ELISA Kit

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Human MX1(Myxovirus Resistance 1) Microsample ELISA Kit [orb2997899]
Reactivity:
Human
Dynamic Range:
1.57-100 ng/mL
Sensitivity:
0.61 ng/mL
Sizes Available: 48 T, 96 T
Human MX1 ELISA Kit [orb562401]
Reactivity:
Human
Dynamic Range:
0.313-20ng/ml
Sensitivity:
0.188ng/ml
Sizes Available: 96 T, 48 T
MF-Human MX1(Myxovirus Resistance 1) ELISA Kit [orb3203533]
Reactivity:
Human
Dynamic Range:
0.31-20ng/mL
Sensitivity:
0.17ng/mL
Sizes Available: 48 T, 96 T
FS-Human MX1(Myxovirus Resistance 1) ELISA Kit [orb3197196]
Reactivity:
Human
Dynamic Range:
0.31-20ng/mL
Sensitivity:
0.18ng/mL
Sizes Available: 48 T, 96 T
Human MX1 ELISA Kit [orb440984]
Reactivity:
Human
Dynamic Range:
0.781-50 ng/mL
Sensitivity:
0.27 ng/mL
Sizes Available: 48 T, 96 T