Ku70 antibody [N3H10]
Referencia GTX23114-500ul
embalaje : 500ul
Marca : Genetex
Contact local distributor :
Teléfono : +1 850 650 7790
5
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | N3H10 |
Isotype | IgG2b |
Application | WB, ICC/IF, IHC-P, IP |
Reactivity | Human, Mouse, Hamster, Monkey, Xenopus laevis |
| Package | 500 μl |
Datasheet File View product citations for antibody GTX23114 on CiteAb
APPLICATION
Application Note
Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 2μg/mg of protein lysate (native and denatured). Western blotting: use at a concentration of 0.25 - 0.5μg/ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2μg/ml for 30 minute at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min prior to immunostaining. By Western blot, this antibody detects a band of 70 kDa, which corresponds to the predicted molecular weight of Ku 70.
Calculated MW
70 kDa. ( Note )
Positive Control
BT474, HEP-G-2 or HeLa cells. Tonsil tissue sections.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.2% BSA
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human placental extract designated as PSE1-PL.
Purification
Protein A purified
Conjugation
Unconjugated
RRID
AB_367103
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA
Cellular Localization
Nucleus
Background
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
Database
Application Reference
Lin RC et al. Oncol Rep 2023; 50 (3) Polo?like kinase 1 selective inhibitor BI2536 (dihydropteridinone) disrupts centrosome homeostasis via ATM?ERK cascade in adrenocortical carcinoma.
Xiao H et al. Cells 2022; 11 (13) Increased Resection at DSBs in G(2)-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
Application : WB
Reactivity : Human
Soni A et al. DNA Repair (Amst) 2020; 89 : 102828 Chromosome breaks generated by low doses of ionizing radiation in G2-phase are processed exclusively by gene conversion.
Application : WB
Reactivity : Hamster
Mladenov E et al. Sci Rep 2019; 9 (1) : 8255 Radiation-dose-dependent functional synergisms between ATM
Patidar PL et al. Nucleic Acids Res 2016; 44 (4) : 1718-31 The Kub5-Hera/RPRD1B interactome: a novel role in preserving genetic stability by regulating DNA mismatch repair.
Submit a Reference Application : WB
Reactivity : Human
REVIEW
Ku70 antibody [N3H10] Cat. No. GTX23114
| Rating | | ( Average 5 based on 5 users reviews) |
| Western Blot(WB) | | ( Average 5 based on 4 users reviews) |
| Immunocytochemistry/ Immunofluorescence(ICC/IF) | | ( Average 5 based on 1 users reviews) |
![Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114")
Application Reference
Lin RC et al. Oncol Rep 2023; 50 (3) Polo?like kinase 1 selective inhibitor BI2536 (dihydropteridinone) disrupts centrosome homeostasis via ATM?ERK cascade in adrenocortical carcinoma.
Xiao H et al. Cells 2022; 11 (13) Increased Resection at DSBs in G(2)-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
Application : WB
Reactivity : Human
Soni A et al. DNA Repair (Amst) 2020; 89:102828 Chromosome breaks generated by low doses of ionizing radiation in G2-phase are processed exclusively by gene conversion.
Application : WB
Reactivity : Hamster
Mladenov E et al. Sci Rep 2019; 9 (1):8255 Radiation-dose-dependent functional synergisms between ATM
Patidar PL et al. Nucleic Acids Res 2016; 44 (4):1718-31 The Kub5-Hera/RPRD1B interactome: a novel role in preserving genetic stability by regulating DNA mismatch repair.
Application : WB
Reactivity : Human
Morales JC et al. PLoS Genet 2016; 12 (7):e1006107 XRN2 Links Transcription Termination to DNA Damage and Replication Stress.
Application : WB
Reactivity : Human
Yang CC et al. F1000Research 2014; Systems analysis of the prostate tumor suppressor NKX3. 1 supports roles in DNA repair and luminal cell differentiation
Application : WB
Reactivity : Human
Morales JC et al. Nucleic Acids Res 2014; Kub5-Hera, the human Rtt103 homolog, plays dual functional roles in transcription termination and DNA repair.
Application : WB
Reactivity : Mouse
You BJ et al. Food Chem Toxicol 2014; 65:205-12 Non-homologous end joining pathway is the major route of protection against 4β-hydroxywithanolide E-induced DNA damage in MCF-7 cells.
Application : WB
Reactivity : Human
| SDS | |
|---|---|
| PBS.pdf | |
| Sodium Azide.pdf | |