ASM10061-STR-100 | LAMP2 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7 (ASM10061). Tissue: Corneal Endothelial Cell (CEC). Species: Rabbit. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody (ASM10061) at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green). Courtesy of: Eunduck E.P. Kay, Doheny Eye Institute.
Western Blot analysis of Human, Mouse HEK293 and 3T3NIH cell lysates showing detection of ~100-110 kDa LAMP2 protein using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7 (ASM10061). Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 µg). Lane 3: Mouse 3T3NIH lysate (10 µg). Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody (ASM10061) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rat at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~100-110 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC/IF, IP
Primary Accession	P17047
Other Accession	NP_001017959.1
Host	Rat
Isotype	IgG1
Reactivity	Human, Mouse, Rabbit
Clonality	Monoclonal
Description	Rat Anti-Mouse LAMP2 Monoclonal IgG1
Target/Specificity	Detects ~100-110kDa.
Other Names	CD107b Antibody, Igp110 Antibody, Igp2 Antibody, Lamp2C Antibody, LampB Antibody, MAC3 Antibody, Lysosome-associated membrane glycoprotein 2 Antibody, LAMP-2 Antibody, Lysosome-associated membrane protein 2 Antibody, CD107 antigen-like family member B Antibody, Lysosomal membrane glycoprotein type B Antibody, LGP-B Antibody
Clone Names	GL2A7
Immunogen	Purified preparation of mouse liver lysosomal membranes
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-141 was sufficient for detection of LAMP2 in 20 µg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cell Membrane \| Endosome \| Lysosome \| Endosome membrane \| Lysosome membrane

LAMP2 Antibody