Lysozyme ELISA Kit (Chicken)
Referencia OKCA00263
embalaje : 96Wells
Marca : Aviva Systems Biology
Predicted Species Reactivity | Chicken | ||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology Lysozyme ELISA Kit (Chicken) (OKCA00263) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Chicken Lysozyme antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Lysozyme and incubated. The Lysozyme found in the sample or standards competes with the biotinylated Lysozyme for limited binding sites on the immobilized anti-Chicken Lysozyme antibody. Excess unbound biotinylated Lysozyme and sample or standard Lysozyme is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Lysozyme captured in the well and inversely proportional to the amount of Lysozyme which was contained in the sample or standard. | ||||||||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Range | 15.625 ng/mL - 4,000 ng/mL | ||||||||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Reproducibility |
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ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||||||||||||||||
Sample Type | Serum, Plasma, Egg white | ||||||||||||||||||||||||||||||||||||||||||||||||
Sensitivity | 14.876 ng/ml | ||||||||||||||||||||||||||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of chicken lysozyme. No significant cross-reactivity or interference between chicken lysozyme and analogues was observed. | ||||||||||||||||||||||||||||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive Immunoassay |
Gene Symbol | Lysozyme |
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