NeoPure™ Total RNA Extraction Kit
Description
NeoPure Total RNA Extraction Kit from Neo Biotech provides a fast and efficient purification method for isolating high-quality total RNA from as few as 15 cells up to 2 million cells.
The isolated RNA is highly pure and ready for downstream applications, including real-time RT-PCR, RNA-seq, and other enzymatic reactions.
The kit enables co-purification of short and long RNA, including messenger RNA (mRNA), ribosomal RNA (rRNA), microRNA (miRNA), and other small RNAs, in the same extraction.
Available in bottle format, including a 96-test format, the kit supports manual extraction and is fully automatable on a variety of extraction instruments.
Key Benefits
- No genomic DNA contamination.
- Quantitative RNA recovery: from 15 to 2,000,000 cells.
- Ultra-pure RNA: eluates free of PCR contaminants and ready for downstream applications.
- Reduced plastic use: magnetic bead-based technology enables up to 50% reduction in plastic consumption.
- Rapid: 85-minute protocol for manual and automated extraction.
- Easy storage: room temperature (15–25 °C).
- Automation compatible: suitable for the most common automated extraction systems.
Compatible Automated Systems
- Allsheng Auto-Pure96, Auto-Pure32A, and Auto-Pure Mini.
- Procomcure Phoenix-Pure96/32.
- MOLGEN PurePrep 96/32.
- BIOER GenePure Pro NPA-32P.
- MGI SP-NE32.
- BIGFISH BFEX 32.
- 3D MED ANDiS 350 and other extraction systems.
Product Properties
NeoPure Total RNA Extraction Kit — Bottle Format
| Property | Details |
|---|---|
| Description | Isolation of RNA from as few as 15 cells up to 2 million cells. |
| Target | Total RNA, mRNA, miRNA, and rRNA. |
| Features | Validated for isolation of total RNA from cultured mammalian cells. For research use only (RUO). Room-temperature storage (15–25 °C). Magnetic bead separation technology. Pure RNA. Available in bottle format. Compatible with the most common automated systems. Extraction time: 85 minutes for 96, 32, or 16 samples. |
| Technology | Magnetic beads. |
| Sample material | Cultured mammalian cells. |
| Sample amount | 15–2,000,000 cells. |
| Elution volume | 30–100 µL. |
| Processing mode | Manual and automated. |
| Typical downstream applications | RT-PCR/PCR, qPCR, qRT-PCR, real-time PCR, real-time RT-PCR, automated fluorescent DNA sequencing, next-generation sequencing (NGS). |
| Storage temperature | Room temperature (15–25 °C). |
Formats and Components
Bottle format: 10 tests or 96 tests
- NeoPure Total Lysis Buffer 1.
- NeoPure Total Magnetic Beads.
- NeoPure Total Washing Buffer 1G.
- NeoPure Total Washing Buffer 2G.
- NeoPure Total Elution Buffer 1.
- NeoPure Total Reductant.
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