Norethindrone ['68-22-4]

Biological Activity

Norethindrone is a synthetic progestin, which mimic the actions of the endogenous ovarian hormone progesterone.

Customer Product Validations & Biological Datas

	Source	J Clin Pharmacol (2012). Figure 1. Norethindrone
	Method	PK and Pharmacodynamic (PD) Assessments
	Cell Lines
	Concentrations	0.5 mg/kg
	Incubation Time	1, 7, 14, 21, 22, 28, 35, 42, 49, 50, and 56 days
	Results	Mean steady-state Cmax, AUC, and Cmin and their 90% CIs of their GLSMRs fell within the no-effect limit boundary of 0.80 to 1.25.

Protocol (for reference only)

Cell Experiment
Cell lines	Human epithelial cervical cancer cells
Preparation method	Thereafter, the cells were incubated with serum-free, phenol-free DMEM for 2 hrs, before treatment with 100 nM DEX, MPA, P4 and NET-A for 1 hr. Cells were crosslinked for 10 min at 37°C with1% formaldehyde and the reaction was stopped with 0.1 mM glycine for 5 min, shaking at room temperature.
Concentrations	100 nM
Incubation time	1 h

Animal Experiment
Animal models	Female Sprague-Dawley rats
Formulation	corn oil
Dosages	3 μg/rat/day
Administration	oral

Chemical Information

Molecular Weight	298.42
Formula	C20H26O2
CAS Number	68-22-4
Form	Solid
Solubility (25°C)	DMSO 50 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Africander DJ, et al. J Steroid Biochem Mol Biol. A comparative study of the androgenic properties of progesterone and the progestins, medroxyprogesterone acetate (MPA) and norethisterone acetate (NET-A).

[2] Govender Y, et al. PLoS One. The injectable-only contraceptive medroxyprogesterone acetate, unlike norethisterone acetate and progesterone, regulates inflammatory genes in endocervical cells via the glucocorticoid receptor.