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O6-Benzylguanine [19916-73-5]

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Referencia NB-64-41830-50mg

embalaje : 50mg

Marca : Neo Biotech

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O6-Benzylguanine

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Synonyms:

Catalog No. T7539 Copy Product Info

Purity: 98.90%

O6-Benzylguanine is a guanine analog with antineoplastic activity,and is a potent O6-alkylguanine DNA alkyltransferase (AGT) inactivator.

Cas No. 19916-73-5

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.90%

Appearance:Solid

Color:White

Product Information

O6-Benzylguanine AI Summary

O6-Benzylguanine exhibits significant inhibitory activity against O6-alkylguanine-DNA alkyltransferase (AGT), also known as O6-methylguanine-DNA methyltransferase (MGMT), demonstrating potential as a therapeutic agent in cancer treatment, particularly breast cancer and colorectal carcinoma. In various cell lines, the compound shows effective inhibition with low IC50 and ED50 values, such as an ED50 of 0.2 µM and 0.05 µM in HT-29 cell-free extracts and HT-29 cells respectively. In MCF-7 breast cancer cells, it removes O6-methylguanine from DNA most effectively at 0.1 µM. Additionally, O6-Benzylguanine enhances the cytotoxicity of ACNU towards HeLa S3 cells with notable dose-modifying effects. Despite reduced effectiveness against certain AGT mutants, it maintains significant inactivation capacity in the presence of DNA. Beyond AGT inhibition, O6-Benzylguanine also displays bioactivity against the SARS-CoV-2 virus, inhibiting virus-induced cytotoxicity in VERO-6 cells, and it demonstrates weak myeloperoxidase inhibition while affecting multiple signaling and enzymatic pathways. Its inhibitory effects extend to aldehyde oxidase-mediated drug metabolism and myeloperoxidase activity, indicating broader pharmacological potential..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	O6-Benzylguanine is a guanine analog with antineoplastic activity,and is a potent O6-alkylguanine DNA alkyltransferase (AGT) inactivator.

Chemical Properties

Molecular Weight	241.25
Formula	C₁₂H₁₁N₅O
Cas No.	19916-73-5
Smiles	O(CC1=CC=CC=C1)C2=C3C(=NC(N)=N2)N=CN3
Relative Density.	1.48 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Keep away from direct sunlight, Pure form: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 245 mg/mL (1015.54 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: < 10 mg/mL (41.45 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 10 mg/mL (41.45 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.1451 mL	20.7254 mL	41.4508 mL	207.2539 mL
5 mM	0.8290 mL	4.1451 mL	8.2902 mL	41.4508 mL
10 mM	0.4145 mL	2.0725 mL	4.1451 mL	20.7254 mL
20 mM	0.2073 mL	1.0363 mL	2.0725 mL	10.3627 mL
50 mM	0.0829 mL	0.4145 mL	0.8290 mL	4.1451 mL
100 mM	0.0415 mL	0.2073 mL	0.4145 mL	2.0725 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

transcriptionalRNASynthesisRNA SynthesisPARP1pancreaticp53p21O6-BenzylguanineO-6-BenzylguanineO6BenzylguanineO6-alkylguanine-DNA-alkyltransferasO6 BenzylguanineInhibitorinhibitguanineDNASynthesisDNAAlkylationDNA/RNA SynthesisDNA SynthesisDNA AlkylationcyclincancerApoptosisantineoplasticanalog

Related Tags: O6-Benzylguanine chemical structure | O6-Benzylguanine formula | O6-Benzylguanine molecular weight