ASM10398-STR-400 | p33 ING1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p33 ING1 Polyclonal Antibody (ASM10398). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p33 ING1 Polyclonal Antibody (ASM10398) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p33 ING1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
Western blot analysis of Mouse brain cell lysates showing detection of ING1 protein using Rabbit Anti-ING1 Polyclonal Antibody (ASM10398). Primary Antibody: Rabbit Anti-ING1 Polyclonal Antibody (ASM10398) at 1:1000. Left: cell expressed flag-tag. Right: Untagged.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p33 ING1 Polyclonal Antibody (ASM10398). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p33 ING1 Polyclonal Antibody (ASM10398) at 1:50 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p33 ING1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC/IF
Primary Accession	Q9QXV3
Other Accession	NP_036049.2
Host	Rabbit
Reactivity	Human, Mouse
Clonality	Polyclonal
Description	Rabbit Anti-Human p33 ING1 Polyclonal
Target/Specificity	Detects ~33kDa.
Other Names	Growth inhibitor ING1 Antibody, ING1 Antibody, inhibitor of growth 1 Antibody, p24ING1c Antibody, p33 Antibody, p33ING Antibody, p33ING1b Antibody, p33ING1c Antibody, p47 Antibody, p47ING1a Antibody, tumor suppressor ING1 Antibody
Immunogen	Mouse p33 ING1 N-terminal peptide –KLH conjugates
Purification	Protein A Purified
Storage	-20ºC
Storage Buffer	TBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	0.25 mg/ml was sufficient for detection of SPC-145 in lysates prepared from human melanoma cell lines by western blot analysis.
Cellular Localization	Nucleus

p33 ING1 Antibody