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> Recombinant Human Bone morphogenetic protein 2 protein(BMP2) (Active)

Recombinant Human Bone morphogenetic protein 2 protein(BMP2) (Active)

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Referencia CSB-AP002981HU-2ug

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CSB-AP002981HU
Abbreviation Recombinant Human BMP2 protein (Active)
MSDS
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  • CSB-AP002981HU SDS-page

Product Details

Purity
≥95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of >5.0x103 IU/mg.
Target Names
Uniprot No.
Research Area
Cancer
Alternative Names
BDA2; BMP-2; BMP-2A; Bmp2; BMP2_HUMAN; BMP2A; Bone morphogenetic protein 2; Bone morphogenetic protein 2A
Species
Homo sapiens (Human)
Source
E.Coli
Expression Region
283-396aa
Complete Sequence
M+QAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
Mol. Weight
13 kDa
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
Form
Liquid or Lyophilized powder
Buffer
Lyophilized from a 0.2 µm filtered concentrated solution containing 10 mM sodium citrate pH 3.5.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description

BMP2 is a critical osteoinductive growth factor whose signaling through SMAD-dependent pathways drives mesenchymal stem cell commitment toward the osteoblast lineage, making validated recombinant forms essential for differentiation research and tumor biology models. This tag-free recombinant human BMP2 encompasses the full mature protein (residues 283–396) and demonstrates an ED50 below 200 ng/mL in an ATDC5 alkaline phosphatase induction assay, corresponding to a specific activity exceeding 5.0 × 10³ IU/mg — confirming robust receptor-binding potency suitable for osteogeni...

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Target Background

Function
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can...
Gene References into Functions
  1. These results not only call into question the use of VEGFA alone in bone regeneration, but also highlight the importance in BTE of appropriately formulated combined delivery of VEGFA and BMP2.
  2. This work indicates that NELL-1, HMGB1, and CCN2 might enhance bone defect healing via the recruitment of endogenous cells and induction of vascularization and act via different processes than BMP2.
  3. Serum BMP2 and Smad4 levels in patients with senile osteoporotic fracture were significantly lower than those in normal controls
  4. conclude that SUMO3-tagged hBMP2 is more suited for generation of soluble form of the protein and addition of SUMO3 tag does not affect the functional activity of hBMP2
  5. The present study identified a change in miR-22, miR-140, and BMP-2 expression in the synovial fluid of patients with osteoarthritis before and after arthroscopic debridement.
  6. The study revealed an enhanced sensitivity of aortic valve interstitial cells to osteogenic inductors in aortic stenosis patients, which indicates probable implication of OPN, OPG, and BMP2 genes in pathogenesis of aortic valve calcification.
  7. The rhBMP2 monomer and dimer were eluted at 0.9 M and 0.6 M NaCl, respectively. The alkaline phosphatase assay of rhBMP2 (0, 50, 100, 200, and 400 ng/ml) was analyzed on C2C12 cells and maximum 200 ng/ml activity was observed in dose dependent manner
  8. In contrast to BMP-2, BMP-7 concomitantly inhibited the expression of profibrotic genes
  9. The binding free energies indicate that ALK-3 preferably binds to BMP-2 instead of BMP-9. The structural analysis shows that ALK-3 binding with BMP-2 occurs in a perfectly symmetry pathway, whereas this symmetry is lost for possible ALK-3 interactions with BMP-9
  10. The results demonstrate the efficacy of HPP-GC hydrogel in minimizing the diffusive loss of rhBMP-2 from the implantation site, compared to the collagen hydroxyapatite scaffold. PMID:

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