RH GM-CSF
Referencia 100-08-20UG
embalaje : 20UG
Marca : FUJIFILM
Recombinant Human GM-CSF
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection. Human and mouse GM-CSF show no cross-reactivity.
Also available as a GMP product: CTGrade GMP rh GM-CSF
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Prominent Band 14.6 kDa
Figure 1. rh GM-CSF (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 14.6 kDa.
Cell-Based Proliferation Assay
Figure 2. Recombinant Human GM-CSF induced proliferation in TF-1 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | CSF-2, Pluripoietin-α, MGI-1GM |
| Accession Number | P04141 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 14.6 kDa (128 aa) |
| AA Sequence | MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS-PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | TF-1 cell proliferation | ≤ 0.2 ng/mL; ≥ 5.0 x 106 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.