StorageThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).
Application Details
Detect Range:Request Information Sensitivity:Request Information Sample Type:Serum, Plasma, Other biological fluids Sample Volume: 1-200 μL Assay Time:1-4.5h Detection wavelength:450 nm
Detection Method:Sandwich Test principle:This assay employs a two-site sandwich ELISA to quantitate MAPKAP1 in samples. An antibody specific for MAPKAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Target of rapamycin complex 2 subunit MAPKAP1 is a protein similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. The deduced 522-amino acid protein has a nuclear localization signal, 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. Comparison of SIN1 with homologs from various species revealed a short, highly conserved region, designated Box1, located within a larger conserved domain, designated CRIM (conserved region in middle).
If you have published an article using product EK9914, please notify us so that we can cite your literature.
YES