SynBlock ELISA Blocking Buffer
Referencia MBS258400-5x1L
embalaje : 5x1L
Marca : MyBiosource
SynBlock ELISA Blocking Buffer Accessory Reagent – Supplied at 1X.
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258400Description: Eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. SynBlock ELISA Blocking Buffer provides superior background-blocking performance without the use of conventional… More details
Product Overview
Online Datasheet in PDFProduct Name
SynBlock ELISA Blocking Buffer, Accessory Reagent
Full Product Name
SynBlock ELISA Blocking Buffer
Product Synonym Names
SynBlock ELISA Blocking Buffer
Form/Format
Supplied at 1X.
Preparation and Storage
Store at 2-8 degree C.
pH
7.4 at 1X
Contains
All synthetic materials.
Product Categories/Family for SynBlock ELISA Blocking Buffer accessory reagent
ELISA Solutions; Blocking Buffers
Application Notes for SynBlock ELISA Blocking Buffer accessory reagent
1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
Other Notes
Small volumes of SynBlock ELISA Blocking Buffer accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SynBlock ELISA Blocking Buffer purchase
MBS258400 is an accessory reagent targeting SynBlock ELISA Blocking Buffer.