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> ULK1 Antibody

ULK1 Antibody

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Referencia ASM10520-A655-100

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  • ICC/IF - ULK1 Antibody ASM10520
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK1 Antibody (D) Composite.
  • WB - ULK1 Antibody ASM10520
    Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 112.6 kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.6 kDa.
  • WB - ULK1 Antibody ASM10520
    Western blot analysis of Rat Brain cell lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession O75385
Other Accession NP_003556.1
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human ULK1 Polyclonal
Target/Specificity Detects ~100 kDa.
Other Names Unc-51 like kinase 1 (C. elegans) Antibody, Autophagy related protein 1 homolog Antibody, Autophagy-related protein 1 homolog Antibody, FLJ38455 Antibody, Serine/threonine-protein kinase ULK1 Antibody, Unc51.1 Antibody, ATG1A Antibody, UNC51, C. elegans, homolog of Antibody, EC:2.7.11.1 Antibody, Serine/threonine protein kinase Unc51.1 Antibody, FLJ46475 Antibody, ATG 1 Antibody, hATG1 Antibody, ATG1 Antibody, Unc-51-like kinase 1 Antibody, Serine/threonine protein kinase ULK1 Antibody, ULK1 Antibody, Unc 51 like kinase 1 Antibody, KIAA0722 Antibody, UNC 51 Antibody, ULK 1 Antibody, ULK1_HUMAN Antibody, UNC51 Antibody, Unc 51 (C. elegans) like kinase 1 Antibody, ATG1 autophagy related 1 homolog Antibody,
Immunogen Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cytosol | Preautophagosomal Structure