ASM10534-P594-100 | WDFY3 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody (ASM10534). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody (ASM10534) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 395.2 kDa WDFY3 protein using Rabbit Anti-WDFY3 Polyclonal Antibody (ASM10534). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody (ASM10534) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 395.2 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	Q8IZQ1
Other Accession	NP_055806.2
Host	Rabbit
Reactivity	Human, Rat
Clonality	Polyclonal
Description	Rabbit Anti-Human WDFY3 Polyclonal
Target/Specificity	Detects ~400 kDa.
Other Names	WDFY3 Antibody, Alfy Antibody, WD repeat and FYVE domain-containing protein 3 Antibody, Autophagy-linked FYVE protein Antibody, KIAA0993 Antibody
Immunogen	Synthetic peptide from the N-terminal of Human WDFY3 (aa. 533-544)
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-666 was sufficient for detection of WDFY3 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Nucleus Membrane \| Cytoplasm \| Nucleus \| Pml Body \| Membrane \| Peripheral Membrane Protein \| Cytoplasmic Side

WDFY3 Antibody