Sulfadimidine (SM2) ELISA BioAssay™ Kit

Referencia 376914-48T

embalaje : 48Tests

Marca : US Biological

Contact local distributor :


Teléfono : +1 850 650 7790


Applications
E

Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Sulfadimidine (SM2) is synthetic antibacterial and plays an important role in controlling and treatment livestock and poultry diseases. Sulfadimidine (SM2) has become a threat for human health and affected the export of animal derived food due to Sulfadimidine (SM2) residues for abuse, not abiding by withdrawal time, entering into the body and ecological system by food chain. Sulfadimidine (SM2) residue limit is 100mg/kg according to No.235 document of the Ministry of Agriculture of the People's Republic of China. This BioAssay™ kit is a detection product developed based on ELISA technology, with operation time as short as 50 minutes.

Sample Type:
Urine, tissue, milk

Intended Use:
This BioAssay™ kit allows for the in vitro quantitative determination of Sulfadimidine (SM2) concentrations in urine, milk, tissue (chicken, porcine, liver).

Sensitivity:
1ppb

Range:
1-81ppb

Precision:
Intra-assay: CV%<10%
Inter-assay: CV%<10%

Test Principle:
This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay™ kit has been pre-coated with Sulfadimidine (SM2) antigen. Standards or samples are added to the appropriate microtiter plate wells with Sulfadimidine (SM2) specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Sulfadimidine (SM2) and Sulfadimidine (SM2) in standards or samples with the Sulfadimidine (SM2) special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Sulfadimidine (SM2) in the standards or samples. The color development is stopped and the intensity of the color is measured.

Kit Components:
Assay plate, 96T/48T
Standard (96T), 6x1ml
Standard (48T), 6x0.5ml
HRP-conjugate (96T), 1x7ml
HRP-conjugate (48T), 1x3.5ml
Antibody (96T), 1x7ml
Antibody (48T), 1x1x3.5ml
TMB Substrate (96T), 1x12ml
TMB Substrate (48T), 1x6ml
Stop Solution (96T), 1x10ml
Stop Solution, (48T), 1x5ml
Sample Diluent (20x) (96T), 2x20ml
Sample DIluent (20x) (48T), 1x20ml
Wash Buffer (10X) (96T), 1x50ml
Wash Buffer (10X) (48T), 1x25ml
Adhesive Strip (96T/48T), 4x strips
Instruction Manual (96T/48T), 1x manual

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C.
Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.