Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay™ Kit

Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. Thiobarbituric Acid Reactive Substances (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery. TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (lex/em=560nm/585nm) is directly proportional to TBARS concentration in the sample.

Sensitive and accurate. Linear detection range
Colorimetric Assay: 1-30uM MDA
Fluorometric Assay: 0.1-1.5uM MDA

Direct Assays: Serum, plasma, urine, saliva and other biological samples
Drug Discovery/Pharmacology: Effects of drugs on TBARS.

T3774-92A: TBA Reagent, 1x25ml
T3774-92B: Trichloroacetic Acid, 10%, 1x25ml
T3774-92C: 6M MDA Standard, 1x1vial

Store all reagents at -20°C. Avoid freeze/thaw cycles. Stable for 6 months after receipt.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.