Human AMY1A (Alpha-amylase 1A) CLIA Kit

Referencia NB-22-40401-1

embalaje : 96T

Marca : Neo Biotech

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Teléfono : +1 850 650 7790

  • Human AMY1A (Alpha-amylase 1A) CLIA Kit

General Info

Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This human AMY1A kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence

Information

Gene Symbol: AMY1A
Gene ID: 276/277/278
Uniprot ID: AMY1A_HUMAN
Specificity: This kit recognizes Human AMY1 in samples. No significant cross-reactivity or interference between Human AMY1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids

Description

Function Calcium-binding enzyme that initiates starch digestion in the oral cavity. Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins.
Protein Name Alpha-Amylase 1a
1 -4-Alpha-D-Glucan Glucanohydrolase 1
Salivary Alpha-Amylase
Database Links Reactome: R-HSA-189085
Cellular Localisation Secreted
Alternative CLIA Names Alpha-Amylase 1a CLIA kit
1 -4-Alpha-D-Glucan Glucanohydrolase 1 CLIA kit
Salivary Alpha-Amylase CLIA kit
AMY1A CLIA kit
AMY1 CLIA kit
Specificity This kit recognizes Human AMY1 in samples. No significant cross-reactivity or interference between Human AMY1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 92
EDTA plasma(n=8) 95-111 103
Cell culture media(n=8) 87-98 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.92 1.76 8.22 0.97 1.83 8.71
Standard deviation 0.10 0.13 0.55 0.08 0.14 0.54
CV (%) 10.87 7.39 6.69 8.25 7.65 6.20
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance