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> ERp57 (GRP58) Antibody

ERp57 (GRP58) Antibody

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Referencia ASM10103-STR-200

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  • ICC/IF - ERp57 (GRP58) Antibody ASM10103
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 (Grp58) Monoclonal Antibody, Clone Map.ERP57 (ASM10103). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Erp57 (Grp58) Monoclonal Antibody (ASM10103) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erp57 (Grp58) Antibody. (C) Composite.
  • IHC - ERp57 (GRP58) Antibody ASM10103
    Immunohistochemistry analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (ASM10103). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (ASM10103) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis and Hair Follicles.
  • WB - ERp57 (GRP58) Antibody ASM10103
    Western Blot analysis of Human cell lysates showing detection of Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (ASM10103). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (ASM10103) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • ICC/IF - ERp57 (GRP58) Antibody ASM10103
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (ASM10103). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (ASM10103) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and perinuclear staining.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP
Primary Accession P30101
Other Accession NP_005304.3
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Bovine, Guinea Pig, Dog
Clonality Monoclonal
Description Mouse Anti-Human ERp57 (GRP58) Monoclonal IgG1
Target/Specificity Detects ~57kDa.
Other Names ERp60 Antibody, ERp61 Antibody, Grp57 Antibody, Grp58 Antibody, P58 Antibody, PDIA3 Antibody, PI PLC Antibody, 58 kDa glucose regulated protein antibody, 58 kDa glucose-regulated protein antibody, 58 kDa microsomal protein antibody, Disulfide isomerase ER 60 antibody, Disulfide isomerase ER-60 antibody, Endoplasmic reticulum resident protein 57 antibody, Endoplasmic reticulum resident protein 60 antibody, ER p57 antibody, ER protein 57 antibody, ER protein 60 antibody, ERp 57 antibody, ERp57 antibody, Glucose Regulated Protein 58 Kd antibody, GRP 57 antibody, GRP 58 antibody, GRP57 antibody, HsT17083 antibody, p58 antibody, PDIA 3 antibody, PDIA3 antibody, PDIA3_HUMAN antibody, Phospholipase C alpha antibody, PI PLC antibody, Protein disulfide isomerase A3 antibody, Protein disulfide isomerase family A member 3 antibody, Protein disulfide-isomerase A3 antibody
Clone Names Map.ERp57
Immunogen Human recombinant ERp57 (Grp58)
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.5 µg/ml of SMC-168 was sufficient for detection of ERp57 in 10 µg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Melanosome