Cd19 Mouse Monoclonal Antibody [Clone ID: MB19-1]

CAT#: AM08032AC7-N

Cd19 mouse monoclonal antibody, clone MB19-1, APC-Cy7


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Specifications

Product Data
Clone Name MB19-1
Applications FC
Recommended Dilution Flow Cytometry: < / = 0.2 µg/10e6 cells. (Ref.1-3)
Reactivities Mouse
Host Mouse
Isotype IgA
Clonality Monoclonal
Immunogen CD19+ mouse pre-B cell line, 300.19. (Ref.1)
Specificity This antibody is specific to CD19 B cell differentiation antigen (Mr. 95 kDa).
Formulation PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent.
Label: APC-Cy7
State: Liquid purified Ig fraction.
Label: Allophycocyanin-Cyanine 7
Concentration lot specific
Conjugation APC-Cy7
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e., B220+/CD43+/HSA+) through fully differentiated B cell stages. (Ref.1-7) Terminally differentiated plasma cells do not express CD19.1 In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. (Ref.3,4) All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. (Ref.1,7) Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. (Ref.7)
Synonyms Leu-12, B-cell marker
Reference Data

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