Glutamate Dehydrogenase BioAssay™ Kit (GLDH)

Referencia 172371-100T

embalaje : 100Tests

Marca : US Biological

Contact local distributor :


Teléfono : +1 850 650 7790


Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Glutamate Dehydrogenase (GLDH) is an enzyme which catalyzes the interconversion of glutamate and a-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections.

This non-radioactive, colorimetric GLDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565nm. The increase in absorbance at 565nm is directly proportional to the enzyme activity.

Intended Use:
For quantitative determination of glutamate dehydrogenase activity in plasma, serum, urine, tissue and culture media.

Detection Range:
0.4-80U/L for 30 min reaction.

Detection Limit:
0.1U/L for 120 min reaction.

Kit Components:
172371A: Assay Buffer 1x 10ml
172371B: NAD Solution, 1x1ml
172371C: MTT Solution, 1x1.5ml
172371D: Diaphorase, 1x120ul
172371E: Calibrator, 1x1.5ml
172371F: Substrate (1M Glutamate), 1x1.5ml

Storage and Stability:
Store all components at -20°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.