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> Protocadherin Gamma (pan) Antibody

Protocadherin Gamma (pan) Antibody

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Referencia ASM10288-A565-100

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  • ICC/IF - Protocadherin Gamma (pan) Antibody ASM10288
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone S159-5 (ASM10288). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody (ASM10288) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Nucleus . Magnification: 60X.
  • WB - Protocadherin Gamma (pan) Antibody ASM10288
    Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone S159-5 (ASM10288). Load: 10 µg. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody (ASM10288) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession Q91XZ0
Other Accession NP_291062.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Mouse Protocadherin Gamma (pan) Monoclonal IgG1
Target/Specificity Detects ~100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
Other Names PCDH gamma A Antibody, PCDH-gamma-A Antibody, PCDHGA Antibody, Protocadherin gamma A Antibody, Protocadherin gamma subfamily A Antibody, Gamma Protocadherin A (pan) Antibody, Pan-Gamma-Protocadherin-A Antibody, Pan Gamma Protocadherin A Antibody
Clone Names S159-5
Immunogen Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-454 was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane