Il2rb Rat Monoclonal Antibody [Clone ID: 5H4]

CAT#: AM08078BT-N

Il2rb rat monoclonal antibody, clone 5H4, Biotin

Conjugation: Unconjugated APC Biotin FITC PE


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Specifications

Product Data
Clone Name 5H4
Applications FC
Recommended Dilution Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
Reactivities Mouse
Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Rat myeloma YB2/0 transfected with truncated mouse IL-2RB cDNA. (Ref.1)
Specificity This antibody recognises beta chain (Mr. 90-100 kDa) of the IL-2 Receptor.
It does not block IL-2 binding. (Ref.1)
Formulation PBS containing 0.09% Sodium Azide as preservative.
Label: Biotin
State: Liquid purified Ig fraction.
Concentration lot specific
Conjugation Biotin
Storage Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the alpha chain which binds IL-2 with low affinity; (ii) the beta chain that binds IL-2 with high affinity; and (ii) the common gamma chain (gc) that does not bind IL-2. (Ref.2) The high affinity receptor complex is an alpha/beta/gamma heterotrimer with a Kd of 1.3 x 10e-11 M. (Ref.2) In mouse spleen, CD122 is expresed on ~30% of CD8+ cells and all NK cells, but < 1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. (Ref.4) Cytoplasmic regions of the IL-2R beta chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes. (Ref.1,4)
Synonyms Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL2 Receptor beta, P70-75
Reference Data

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