Biochemicals - IPTG

Biochemicals - IPTG


Isopropyl β-D-1-thiogalactopyranoside, abbreviated by IPTG, is a molecular biology reagent.

This compound is used as an analogue of allolactose, a lactose metabolite that activates the transcription of lactose operon and in particular the β-galactosidase gene, lacZ. Like allolactose or lactose, IPTG binds to the suppressor of the lactose operon, LacI, which prevents its binding to the operator and thus induces the transcription of the β-galactosidase gene. Unlike allolactose, IPTG is not a substrate for this enzyme because the presence of a sulfur atom in IPTG makes the chemical bond with the isopropyl part non-hydrolysable, so IPTG is what is called a free inducer. Not being metabolizable, the concentration of IPTG does not vary in the culture, which makes IPTG induction constant and stable.

In the laboratory, IPTG is used to induce the expression of recombinant proteins under the control of the lake promoter or its derivatives in Escherichia coli. Bacteria carrying a plasmid containing the gene of interest are cultured in the absence of IPTG until cell density reaches a sufficient level. The addition of IPTG to the culture allows the production of the protein to be induced at the desired time.

Resultados de su búsqueda : 171 Producto encontrado

Refine su búsqueda :

RUOCE / IVD
  • Unconjugated 48
  • Biochemicals 89
  • Buffers and reagents 38
  • cell culture products 28
  • Inhibitor/Antagonist/Agonist 11
  • Protein/peptide 2
  • Biological fluids 1
  • Discontinued 1
  • Other products 1
  • Cell culture 28
  • IHC 2
  • WB 2


Referencia
Descripción
Cond.
Precio Sin IVA
HY-15921-1ml
 10mM*1mL 
HY-15921-1g
 1g 
HY-15921-5g
 5g 
HY-15921-500mg
 500mg 
HY-15921-10g
 10g 
HY-15921-25g
 25g 
GF101-01
 1ml 
orb340528-1g
 1g 
orb340528-5g
 5g 
NB-45-00028-20ml
 20ml 
NB-45-00028-5ml
 5ml 
NB-45-00028-100ml
 100ml 
NB-45-00028-5x200ul
 1ml 
NB-45-00030-25g
 25g 
NB-45-00030-5x5g
 5x5g 
NB-45-00111-5g
 5g 
NB-45-00030-5g
 5g