High troughput kits
Transformation of normal cells into neoplastic cells occurs through a series of genetic and epigenetic alterations, resulting in a cell population capable of proliferating independently of internal and external signals that normally restrict their growth. Traditionally, the soft agar colony formation test was used to monitor cell transformation and anchorage-independent growth, with manual counting of proliferated cells after 3-4 weeks of cell growth.
The 384-well Cell Transformation Assay does not involve subjective manual counting of colonies or require a 3-4 week incubation period. Instead cells are incubated only 6- 8 days in a proprietary semisolid agar media before being lysed and detected by the patented CyQuant® GR Dye in a fluorescence plate reader (see Assay Principle below). This format provides a quantitative, high-throughput method to accurately measure cell transformation, while the short incubation time makes it possible to assay cells transiently transfected with oncogenes or siRNA.
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