embalaje : 100assays
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Histone acetyltransferase (HAT) is an enzyme that acetylates conserved lysine on a histone protein by transferring an acetyl group from acetyl CoA. Histone acetylation can increase gene expression. HAT plays important roles in gene transcription, cell proliferation and differentiation.
The HAT Activity Fluorometric Assay Kit provides a sensitive and sensitive way for detection of HAT activity in a variety of samples based on fluorometric method that eliminates radioactivity in traditional assays. The assay utilizes H3 histone peptide and Acetyl CoA as substrates. The active HAT transfers acetyl groups from Acetyl-CoA to the H3 histone peptide, thereby producing two products - acetylated H3 histone peptide and CoA-SH. The CoA-SH reacts with the developer to yield a fluorophore product that can be easily detected using a fluorometer or a fluorescence plate reader at Ex/Em = 535/587 nm. The kit can determine HAT activity as low as 6 mU in a variety of samples.