Images
Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.
Note

Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.

Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.

Cells were plated at 50,000 cells/100uL/well in a 96 well black wall/clear bottom poly-D lysine plate for 5 hours, and then serum deprived for 1 hour. Cells were treated without (control) or with insulin (150 nM), and incubated at 37°C, 5% CO2 incubator for 30 min. At the end of the incubation time, 100 ul of fatty acid mixture was added into the well, and incubated for another 60 min, the fluorescence signal was measured with a FlexStation plate reader using bottom read mode. A: fibroblasts (Control); B: fibroblasts (Insulin); C: adipocytes (Control); D: adipocytes (Insulin). The fluorescence in blank wells with the growth medium is subtracted from the values for the wells with cells. The background fluorescence of the blank wells may vary depending on the sources of the growth media or the microtiter plates.

  • Specification

    Product Description

    Fatty Acid Uptake Assay Kit is used for the measurement of fatty acid uptake.

    Suitable Sample

    Live Cells

    Excitation/<br>Emission (nm)

    485/515 nm

    Detection Method

    Fluorometric

    Instrument Platform

    Fluorescence Microscope, Microplate Readers

    Regulation Status

    For research use only (RUO)

    Storage Instruction

    Store the kit in desiccated environment at -20°C and avoid from light.

    Note

    Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.

  • Applications

    Quantification