Citrate BioAssay™ Kit

Referencia 172366-100T

embalaje : 100Tests

Marca : US Biological

Contact local distributor :


Teléfono : +1 850 650 7790


Shipping Temp
Dry Ice

Storage Temp
-20°C

Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570nm or fluorescence intensity at λex/em=530/585nm is directly proportional to the citrate concentration in the sample.

Intended Use:
For citrate determination in biological samples (e.g. serum, plasma, urine, tissue and culture media).

Key Features:
Fast and sensitive: Linear detection range: 4 to 400uM citrate for
colorimetric assays and 0.5 to 40uM for fluorimetric assays.

Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling
systems for processing thousands of samples per day.

Kit Components:
172366A: Developer, 1x10ml
172366B: Dye Reagent, 1x120ul
172366C: Citrate Standard, 1x500ul
172366D: CL Enzyme, 1x1vial
172366E: ODC Enzyme, 1x120ul

Storage and Stability:
Store all components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.