K2036-100 assays | NAD/NADH Quantitation Colorimetric Kit Clinisciences

Background

Nicotinamide adenine dinucleotide (NAD) is a coenzyme present in all living cells and exists in two forms: NAD⁺ and NADH. NAD plays important roles in energy transforming and redox state of cells or tissues.

The NAD/NADH Quantitation Colorimetric Kit provides a sensitive and convenient way for detection of the intracellular nucleotides: NADH, NAD and their ratio. The NAD Cycling Enzyme Mix specifically recognizes NADH/NAD in an enzyme cycling reaction, which are not required to purify from samples. The enzyme cycling reaction significantly increases the detection specificity and sensitivity. By comparing with standard NADH, NADH or NADt (NAD and NADH) can be easily quantified. The assay specifically detects NAD and NADH, but not NADPH or NADP.

Features & Properties

Features	Fast and convenient Simple procedure; takes ~2 hours. Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.
Shipping	Gel pack
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

NADH/NAD Extraction Buffer NAD Cycling Buffer NAD Cycling Enzyme Mix NADH Developer Stop Solution NADH Standard (MW:664.4)

NAD/NADH Quantitation Colorimetric Kit

Referencia K2036-100 assays

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Marca : ApexBio Technology