New Ultra-Sensitive qPCR Gene Mutation Detection Kits

New Ultra-Sensitive qPCR Gene Mutation Detection Kits

QClamp® Detects Rare and Actionable Cancer Mutations & Fusion Genes with ultrasensitivity
New Technology : Xenonucleic Acid (XNA) Molecular Clamp Technology

QClamp® Gene Mutation Detection Kit is a highly sensitive qPCR assay that provides detection sensitivity of 0.1% or lower. It is ideal for screening rare and actionable somatic mutations in oncogenes. The assay utilizes a sequence-specific clamp (Xeno-Nucleic Acid probe) that suppresses PCR amplification of wild-type DNA template and selectively amplifies only mutant template. The kits provide a rapid, reproducible and affordable solution which employs a simple workflow and PCR machines that are commonly used in research and clinical labs.


ULTRA-SENSITIVE
Ability to detect below 0.1% mutations 
SAMPLE FORMAT
Suitable for liquid biopsy, FFPE tissue and more 
RAPID
Streamlined workflow with 3 hours turnaround time 

Gene
Mutations
CE/IVD
Codons 12, 13, 59, 61, 117, & 146 
Yes
Codons 12, 13, 59, 61, 117 & 146
Yes
Codons 719, Ex19Del, 790, 858 & 861
Yes
Codon 600
Yes
Codons 542, 545 & 1047
Yes
Codon 617
Yes
Codon 816
No

Medical devices for in vitro diagnostic. Read the instructions for use carefully.

Xenonucleic Acid (XNA) Molecular Clamp Technology


DiaCarta scientists have developed innovative new nucleic acid molecular oligomers that hybridize by Watson-Crick base pairing to target DNA sequences yet have a modified chemical backbone. The xenonucleic acid oligomers (figure: Watson-Crick Base Pairing of DNA with cognate XNA) are highly effective at hybridizing to target sequences and can be employed as molecular clamps in quantitative real-time polymerase chain reactions (PCR) or as highly specific molecular probes for detection of nucleic acid target sequences.


FEATURES AND BENEFITS

  • Molecular clamps for qPCR
  • Synthetic oligomers containing natural A, T,C, G or modified nucleosides (15 to 25 nt long)
  • Hydrophilic and neutral backbone (no phosphate group like PNA)
  • Hybridization by Watson-Crick pairing
  • Resistant to any known nucleases
  • Much higher binding affinity
  • DNA binding is independent of salt concentration
  • Large melting temperature differential (ΔTm = 15-20ºC) in single-nucleotide (SNP's) and insertion/deletions (indels) (5-7ºC for natural DNA)

How it works

QClamp® is a revolutionary new way to screen for somatic mutations that utilize a sequence-specific clamp that suppresses PCR amplification of wild type template DNA. QClamp® allows selective PCR amplification of only mutant templates, which allows the detection of mutant DNA in the presence of a large excess of wild-type templates from a variety of samples including FFPE, liquid biopsy, and traditionally challenging cytology samples.


Supporting data

QClamp Detects below 0.1% mutated DNA
The most sensitive technology that could detect below 0.1% mutant in 2 hours.



QClamp HRM for EGFR Mutation
High-resolution melting (HRM) profiles of clinically relevant EGFR mutations generated by the QClamp EGFR Test.