Study of metabolic status variations on living cells

Study of metabolic status variations on living cells

RBD are new biomarkers corresponding to nutrient transporter ligands. They allow the identification and measurement  of metabolic status variations on living cells in healthy or unhealthy conditions.

The plasma membrane represents the interface with the external environment and handles critical functions notably that of managing exchanges between the cytoplasm and the external environment.

For this, nutrients transporters are regulated with great precision to fuel the cell. Long-neglected as research targets due to the lack of tools enabling their detection, these keys players of cell metabolism constitute a source of information regarding the cell's physiological state.

 

Description

RBD (Recepor Binding Domains)

Recombinants RBD derived from retroviral envelope glycoproteins are cell surface ligands of nutrients transporters 

Glut 1 : glucose transporter

ASCT2 : glutamine transporter

PiT1 et PiT2 : phosphate transporter

XPR1 : inorganic phosphate exporter

FLVCR1 : heme exporte

Figure 1 : RBD derived from the HTLV envelope binding the Glut1 glucose transporter on an extracellular loop

 

Product # Cat Description Conditioning
Glut1.RBD Glut1-G100 The Glut1.RBD kit - GFP 100 tests
Glut1-G25 25 tests
Glut1-M100 The Glu1.RBD kit - Mouse Fc 100 tests
Glut1-M25 25 tests

 


METAscreen ®


METAscreen® are RBD panels which allow to quantify the expression of nutrient transporters at the plasma membrane, for multiples applications:

 

Products
METAscreen
METAscreen® SLC profiling kit METAscreen® Phosphate kit METAscreen® Mito Tox Kit METAscreen® Onco kit METAscreen® Red cell kit
Cat # SLC-25 Phos-25 MitoTox-25 Onco-25 Red-25
Conditioning 25 tests
Details

 

Screen for the expression of the 6 corresponding nutrient transporters on your cells, allowing you to identify those which are  of interest for your research topic.

Establish a meaningful cell surface metabolic signature reflecting cell needs to fuel its metabolism, in terms of glucose, glutamine, and phosphate notably. Monitor its variation during drug treatement, differentiation processes etc.
Get an overview of cellular inorganic phosphate homeostasis by monitoring the two ubiquitous iP importers (PiT1 and PiT2) along with the only, recently identifies, phosphate exporter (XPR1) Detect mitochondrial toxicity or a metabolic switch between glycolysis and oxidative phosphorylation by monitoring Glut1 and FLVCR1. Detect and quantify 3 key nutrient transporters involved in cancer cell metabolism. Detect and quantify key nutrient transporters involved in erythroid differentiation.
RBD inclus Glut1, ASCT2 PiT1, PiT2, XPR1, FLVCR1 PiT1, PiT2, XPR1 Glut1, FLVCR1 Glut1, ASCT2, PiT1 Glut1, ASCT2, PiT1, FLVCR1

 

 

Avantages

 

  • Nutrient transporters are key players of metabolism
  • RBD are unmatched ligands, binding transporters at the cell surface where they are active and runs on living cells
  • Consequently this read-out is relevant to cell physiology   
  • RBD/METAscreen® are easy to use throug widely disseminated instruments

 

  • Protocoles validated on immunofluorescence and flow cytometry

 

Applications

 

  • Toxicologie in vitro : METAscreen®  is particularly relevant for predictive in vitro toxicology applications at early stages of drug development. METAscreen® responds to the challenge of the pharmaceutical industry in the early identification of adverse effects of drugs, before entering expensive clinical trials.
  • Contrôle de la qualité fonctionnelle des cellules : METAscreen® allows the identification of stem cell banks (MSCs, NSCs, keratinocytes) that are unable to differentiate correctly or to qualify cells batches regarding various functional traits such as differentiation potential, proliferation etc. METAscreen® signatures distinguish clearly between good versus bad quality banks, and provide a predictive response in record time, with minimal sample manipulation.