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> Anti-PI 3-Kinase p85 alpha (Phosphatidylinositol-PI-3-Kinase) (FITC) Monoclonal Antibody

Anti-PI 3-Kinase p85 alpha (Phosphatidylinositol-PI-3-Kinase) (FITC) Monoclonal Antibody

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Referencia P4186-03-FITC-100ul

embalaje : 100ul

Marca : US Biological

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P4186-03-FITC PI 3-Kinase p85 alpha (Phosphatidylinositol-PI-3-Kinase) (FITC)

Clone Type
Polyclonal
Host
mouse
Source
bovine
Isotype
IgG1
Grade
Affinity Purified
Applications
IHC IP WB
Crossreactivity
Bo Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen
Western Blot: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized mice were fused with cells of the NS1 mouse myeloma cell line.

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2837A (U5)|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Recombinant protein corresponding to the bovine p85 alpha subunit of PI 3 kinase, expressed in Baculovirus.|Specificity: Recognizes the bcr-homology domain of the p85 alpha subunit of bovine PI 3 kinase. Species Crossreactivity: human, mouse, rat and monkey||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein corresponding to the bovine p85 alpha subunit of PI 3 kinase, expressed in Baculovirus.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the bcr-homology domain of the p85 alpha subunit of bovine PI 3 kinase. Species Crossreactivity: human, mouse, rat and monkey
References
1. Reif, K. et al. (1993) Divergent regulation of phosphatidylinositol 3-Kinase P85 alpha and P85 beta isoforms upon T cell activation. J. Biol. Chem. 278: 10780-10788.