Fab Rabbit IgG (H&L) Antibody Rhodamine Conjugated

Specifications

Product Data
Applications	FC, IF
Recommended Dilution	IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000
Reactivities	Rabbit
Host	Donkey
Clonality	Polyclonal
Immunogen	Rabbit IgG whole molecule
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum.  No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration	1.0 mg/mL - lot specific
Conjugation	TRITC
Storage	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Note	Fab Anti-Rabbit IgG Rhodamine Conjugated Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.