RayBio® Human GRO alpha (CXCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human GRO-alpha ELISA Kit
Human GRO-alpha ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 1000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P09341 |
| Gene Id | 2919 |
| Gene Symbols | CXCL1, GRO, GRO1, GROA, MGSA, SCYB1 |
| Protein Name / Synonyms | Growth-regulated alpha protein (C-X-C motif chemokine 1) (GRO-alpha(1-73)) (Melanoma growth stimulatory activity) (MGSA) (Neutrophil-activating protein 3) (NAP-3) [Cleaved into: GRO-alpha(4-73) GRO-alpha(5-73) GRO-alpha(6-73)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
citations
Chemotaxis-driven delivery of nano-pathogenoids for complete eradication of tumors post-phototherapy
- Species:
- Mouse
- Sample Type:
- Tissue Lysate
- Year:
- 2020
- Journal Name:
- Nature Communications
- PubMed ID:
- 32111847
Overexpression of GAB2 in ovarian cancer cells promotes tumor growth and angiogenesis by upregulating chemokine expression
- Species:
- Human
- Sample Type:
- Conditioned Media (Fallopian Tube Secretory Epithelial Cells)
- Year:
- 2016
- Journal Name:
- Oncogene
- PubMed ID:
- 26657155
Altered release of chemokines by phagocytes from fibromyalgia patients: a pilot study
- Species:
- Human
- Sample Type:
- Conditioned Media (Fibromyalgia patient Monocytes/Neutrophils treated with LPS)
- Year:
- 2016
- Journal Name:
- Innate immunity
- PubMed ID:
- 26341115
Human Umbilical Cord-Derived Mesenchymal Stem Cells Improve Learning and Memory Function in Hypoxic-Ischemic Brain-Damaged Rats via an IL-8-Mediated Secretion Mechanism Rather than Differentiation Pattern Induction
- Species:
- Human
- Sample Type:
- Conditioned Media (Human MSC cells)
- Year:
- 2015
- Journal Name:
- Cellular Physiology and Biochemistry
- PubMed ID:
- 25896602
Diagnostic Value of Circulating CXC Chemokines in Non-small Cell Lung Cancer
- Species:
- Human
- Sample Type:
- Serum (Non small cell lung cancer patients vs controls)
- Year:
- 2015
- Journal Name:
- Anticancer Research
- PubMed ID:
- 26637925
OnlineFirst October 27, 2014; doi: 10.1158/1535-7163.MCT-14-0448
- Species:
- Human
- Sample Type:
- Conditioned Media (Non-small cell lung cancer)
- Year:
- 2014
- Journal Name:
- Molecular Cancer Therapeutics
Inhibition of Epithelial CC-Family Chemokine Synthesis by the Synthetic Chalcone DMPF-1 via Disruption of NF-kB Nuclear Translocation and Suppression of Experimental Asthma in Mice
- Species:
- Human
- Sample Type:
- Conditioned Media (A549 adenocarcinomas)
- Year:
- 2014
- Journal Name:
- Mediators of Inflammation
Llama-derived single variable domains (nanobodies) directed against chemokine receptor CXCR7 reduce head and neck cancer cell growth in vivo.
- Species:
- Human
- Sample Type:
- Conditioned Media (22A cells )
- Year:
- 2013
- Journal Name:
- the Journal of Biological Chemistry
- PubMed ID:
- 23979133
Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer
- Species:
- Human
- Sample Type:
- Conditioned Media (PC3, prostate cancer cell lines)
- Year:
- 2012
- Journal Name:
- Molecular Cancer Therapeutics
Interleukin (IL)-17 versus IL-27: opposite effects on tumor necrosis factor--mediated chemokine production in human keratinocytes
- Species:
- Human
- Sample Type:
- Conditioned Media (Normal human epidermal keratinocytes)
- Year:
- 2012
- Journal Name:
- Experimental Dermatology
- PubMed ID:
- 22082171
