Copper Transporting ATPase 1 Antibody

Referencia ASM10232-STR-100

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  • ICC/IF - Copper Transporting ATPase 1 Antibody ASM10232
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (ASM10232). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (ASM10232) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Copper Transporting ATPase 1 Antibody (D) Composite.
  • WB - Copper Transporting ATPase 1 Antibody ASM10232
    Western Blot analysis of Rat Brain Membrane showing detection of ~180 kDa Copper Transporting ATPase 1 protein using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (ASM10232). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (ASM10232) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~180 kDa. Other Band(s): 250kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP
Primary Accession Q04656
Other Accession NP_000043.3
Host Mouse
Isotype IgG2b
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human Copper Transporting ATPase 1 Monoclonal IgG2b
Target/Specificity Detects ~180kDa in rat brain membrane preparations.
Other Names ATP7A Antibody, ATP 7A Antibody, ATPase Cu transporting Antibody, DSMAX Antibody, FLJ17790 Antibody, MC1 Antibody, MC 1 Antibody, MK Antibody, MNK Antibody, OHS Antibody, Copper pump 1 Antibody, Menke Antibody, OTTHUMP00000062077 Antibody, SMAX3 Antibody, ATPase copper transporting alpha polypeptide Antibody, ATPase Cu++ transporting alpha polypeptide (Menkes syndrome) Antibody, Copper transporting ATPase 1 Antibody, Cu++ transporting P type ATPase Antibody, Menkes disease associated protein Antibody, Menkes syndrome Antibody
Clone Names S60-4
Immunogen Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-398 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.
Cellular Localization Endoplasmic Reticulum | Cytoplasm | Golgi Apparatus | Trans-Golgi Network Membrane | Cell Membrane