Superoxide Dismutase (SOD) Assay Kit, BioAssay™

Superoxide Dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide into O2 and H2O2. They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer. SOD assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a WST-1 dye to form a colored product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.

Sensitive and accurate: Linear detection range of 0.05-3U/mL SOD.
Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day

Determination of SOD in blood, cell, tissue and other biological samples.

S8060-07A: Assay Buffer, 1x20ml
S8060-07B: SOD Enzyme, 1x120ul
S8060-07C: Xanthine, 1x600ul
S8060-07D: DIluent, 1x20ml
S8060-07E: XO Enzyme, 1x120ul
S8060-07F: WST-1, 1x600ul

Store at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.