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Clonidine [4205-90-7]

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Référence NB-64-38496-100mg

Conditionnement : 100mg

Marque : Neo Biotech

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Clonidine

(Synonyms: Nexiclon, Kapvay, Catapres) Copy Product Info

Synonyms: Nexiclon, Kapvay, Catapres

Catalog No. T7045 Copy Product Info

Purity: 99.84%

Clonidine (Kapvay) is a centrally active alpha-adrenergic agonist used predominantly as an antihypertensive agent, usually in combination with other agents. Despite wide-scale use for many years, clonidine has not been linked definitively to either serum aminotransferase elevations or clinically apparent liver injury.

Cas No. 4205-90-7

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.84%

Appearance:Solid

Color:White

Product Information

Bioactivity

Description	Clonidine (Kapvay) is a centrally active alpha-adrenergic agonist used predominantly as an antihypertensive agent, usually in combination with other agents. Despite wide-scale use for many years, clonidine has not been linked definitively to either serum aminotransferase elevations or clinically apparent liver injury.
In vitro	Clonidine (0.01, 0.1 or 1 μM) significantly induces CGRP (α and β) mRNA expression in a dose-dependent manner in endothelial cells. Clonidine treatment (1 μM) for 24 h significantly increases the NO level in endothelial cells. NO pathway modulates CGRP production induced by clonidine [2].
In vivo	Clonidine (3-50 μg/kg, i.p.) potently suppresses dopamine efflux in the prefrontal cortex induced by PCP. Pretreatment with the alpha-2A receptor antagonist (BRL-44408) prevents clonidine from suppressing PCP-induced dopamine overflow in the prefrontal cortex [3]. Clonidine (50 μg/kg, i.p.) induces a significant decrease in body temperature of rat lasting 3 hr, with the maximum at 1 hr after administration. An intracerebroventricular pretreatment of rats with neutral doses of phentolamine 15 min before clonidine considerably antagonizes the clonidine-induced hypothermia[1]. In DMSO-pretreated SO rats, clonidine (0.6 μg i.c.) has no effect on blood pressure. However, after central adenosine A1R blockade (DPCPX) in SO rats, clonidine significantly (P < 0.05, one-way ANOVA) reduces blood pressure. In contrast, in DMSO-pretreated ABD rats, clonidine (0.6 μg i.c.) causes a significant reduction in blood pressure; importantly, central A1R blockade (DPCPX pretreatment) does not influence (P > 0.05, one-way ANOVA) clonidine-evoked reduction in blood pressure in ABD rats. In DPCPX-pretreated SO rats and along with the appearance of the hypotensive response, clonidine causes a significant (P < 0.05) increase in the RVLM pERK1/2 level compared with basal or clonidine treatment in DMSO-pretreated SO rats. In a vehicle (DMSO)-pretreated ABD rats, clonidine significantly (P < 0.05) enhances RVLM pERK1/2, and this response is not affected by DPCPX pretreatment [4].
Synonyms	Nexiclon, Kapvay, Catapres

Chemical Properties

Molecular Weight	230.09
Formula	C₉H₉Cl₂N₃
Cas No.	4205-90-7
Smiles	N(C1=C(Cl)C=CC=C1Cl)C=2NCCN2
Relative Density.	1.3946 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Keep away from direct sunlight, Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 2 mg/mL (8.69 mM), Sonication is recommended.

DMSO: 262 mg/mL (1138.68 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (21.73 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.3461 mL	21.7306 mL	43.4613 mL	217.3063 mL
5 mM	0.8692 mL	4.3461 mL	8.6923 mL	43.4613 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.4346 mL	2.1731 mL	4.3461 mL	21.7306 mL
20 mM	0.2173 mL	1.0865 mL	2.1731 mL	10.8653 mL
50 mM	0.0869 mL	0.4346 mL	0.8692 mL	4.3461 mL
100 mM	0.0435 mL	0.2173 mL	0.4346 mL	2.1731 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

α2-adrenergicα2 adrenergic receptorInhibitorinhibitClonidineBeta ReceptoranesthesiaAdrenergicReceptorAdrenergic Receptor

Related Tags: Clonidine chemical structure | Clonidine in vivo | Clonidine in vitro | Clonidine formula | Clonidine molecular weight