PCSK9 ELISA Kit (Human)

Référence OKEH00374

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PCSK9 ELISA Kit (Human) (OKEH00374) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PCSK9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for PCSK9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PCSK9 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 6.5% (n = 20)
ELISA Kit Component
ComponentAmount
PCSK9 Microplate96 Wells (12 x 8 Well strips)
PCSK9 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PCSK9 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human Proprotein convertase subtilisin/kexin type 9
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPCSK9
Gene Full Nameproprotein convertase subtilisin/kexin type 9
Alias Symbolsconvertase subtilisin/kexin type 9 preproprotein;FH3;FHCL3;HCHOLA3;LDLCQ1;NARC1;NARC-1;neural apoptosis regulated convertase 1;Neural apoptosis-regulated convertase 1;PC9;Proprotein convertase 9;proprotein convertase subtilisin/kexin type 9;subtilisin/kexin-like protease PC9.
NCBI Gene Id255738
Protein NameQ8NBP7
Description of TargetProprotein convertase subtilisin/kexin type 9
Uniprot IDhttps://www.uniprot.org/uniprot/Q8NBP7
Protein Accession #NP_777596.2
Nucleotide Accession #NM_174936.3
Protein Size (# AA)692
Molecular Weight74 kDa

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Référence
Description
Cond.
Prix HT
OKEH02858
 96Wells 
OKAG00244
 1plate:12x8-WellMicrostrips