PPARGC1A ELISA Kit (Human)
Référence OKEH00824
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology PPARGC1A ELISA Kit (Human) (OKEH00824) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPARGC1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPARGC1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PPARGC1A captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.5% (n = 20) Mean Inter-assay CV%: < 8.9% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.19 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | PPARGC1A |
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| Gene Full Name | PPARG coactivator 1 alpha |
| Alias Symbols | LEM6;Ligand effect modulator 6;ligand effect modulator-6;L-PGC-1alpha;peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext;peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a;peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT;peroxisome proliferator-activated receptor gamma coactivator 1-alpha;PGC1;PGC-1(alpha);PGC1A;PGC-1alpha;PGC-1-alpha;PGC-1v;PPAR gamma coactivator variant form;PPARgamma coactivator 1alpha;PPARGC1;PPARGC-1-alpha. |
| NCBI Gene Id | 10891 |
| Protein Name | Q9UBK2 |
| Description of Target | Peroxisome proliferator-activated receptor gamma coactivator 1-alpha |
| Uniprot ID | https://www.uniprot.org/uniprot/Q9UBK2 |
| Protein Accession # | NP_037393.1 |
| Nucleotide Accession # | NM_013261.3 |
| Protein Size (# AA) | 798 |
| Molecular Weight | 91 kDa |