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> 2'-Fucosyllactose [41263-94-9]

2'-Fucosyllactose [41263-94-9]

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Cat# HY-N9965-10mg

Size : 10mg

Marca : MedChemExpress


Description

2'-Fucosyllactose (2'-FL) is an oligosaccharide that could be derived from human milk. 2'-Fucosyllactose regulates the expression of CD14, alleviates colitis and regulates the gut microbiome. 2'-Fucosyllactose stimulates T cells to increase IFN-γ production and decreases IL-6, IL-17, and TNF-α production of cytokines[1][2].

IC50 & Target

Target: IFN-γ, IL-6, IL-17, and TNF-α[1]
In Vitro

2'-Fucosyllactose (2'-FL; 0-12 mg/mL; 48 h) suppress cell-associated CD14 expression and to attenuate LPS (100 μg/mL) stimulated IL-8 secretion in T84 cells[1].
2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) ameliorates inflammation induced by bacterial invasion. 2'-Fucosyllactose inhibits ETEC invasion and attenuated the consequent IL-8 secretion[1].
2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) induces macrophage migration inhibitory factor signal pathways that suppress inflammation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2'-Fucosyllactose Related Antibodies

Western Blot Analysis[1]

Cell Line: T84 cells
Concentration: 0, 2, and 4 mg/mL
Incubation Time: 48 hours
Result: Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression.

Western Blot Analysis[1]

Cell Line: T84 and HCT8 cellsT84 and HCT8 cells
Concentration: 2 mg/mL
Incubation Time: 48 hours
Result: Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression.
Inhibited CD14 and NF-κB induction.
Induced iκB and SOCS2 expression and STAT3 phosphorylation.
In Vivo

2'-Fucosyllactose (2'-FL; 100 mg (200 μL); i.g.; daily, for 4 d; C57BL/6 mice with AIEC infection) inhibits AIEC infection and inflammation in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice (8 weeks) with AIEC (uropathogenic E. coli, and adherent-invasive E. coli) infection[1]
Dosage: 100 mg (200 μL)
Administration: Oral gavage; daily, for 4 days
Result: Had colons lengths were closer to normal.
Inhibited the colonisation of the colonic mucosa by O83-positive bacteria.
Decreased CD14 expression, CD14 mRNA levels, IL-6, IL-17 and TNF-α levels in colonic.
Essai clinique
Masse moléculaire

488.44

Formule

C18H32O15
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O[C@H]2[C@H]([C@@H]([C@@H]([C@@H](O2)C)O)O)O
Structure Classification
Initial Source

Human milk
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité
In Vitro: 

H2O : 50 mg/mL (102.37 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0473 mL 10.2367 mL 20.4733 mL
5 mM 0.4095 mL 2.0473 mL 4.0947 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
Références