ACSL6 Antibody
Cat# MBS9605597-0,1mL
Size : 0.1mL
Marca : MyBiosource
Western blot analysis of extracts from COLO cells using ACSL6 antibody., The lane on the left is treated with the antigen-specific peptide.
MBS9605597 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ACSL6 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9605597MBS9605597 is an antibody targeting ACSL6. Also known as ACSL6 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details
Product Overview
Online Datasheet in PDFProduct Name
ACSL6, Polyclonal Antibody
Full Product Name
ACSL6 Antibody
Product Gene Name
anti-ACSL6 antibody
Product Synonym Names
ACS2; Acyl CoA synthetase long chain family member 6; FACL6; Fatty acid Coenzyme A ligase, long chain 6; LACS 6; LACS2; LACS5; Long chain acyl CoA synthetase 6; Long chain fatty acid CoA ligase 6; Long fatty acyl CoA synthetase 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Peptide affinity purification
Concentration
~1mg/ml (lot specific)
Specificity
ACSL6 antibody detects endogenous levels of total ACSL6
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
ACSL6 peptide (MBS9624314) is used for blocking the activity of ACSL6 antibody (MBS9605597)
Molecular Weight
78 kD
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human ACSL6, corresponding to a region within the internal amino acids.
RRID
AB_2836507
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ACSL6 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ACSL6 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
ELISA (peptide): 1:20000-1:40000
The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IF/ICC: 1:100-1:500
ELISA (peptide): 1:20000-1:40000
The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunofluorescene (IF) of anti-ACSL6 antibody
MBS9605597 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Other Notes
Small volumes of anti-ACSL6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for ACSL6. It may not necessarily be applicable to this product.
NCBI Official Full Name
long-chain-fatty-acid--CoA ligase 6 isoform b
NCBI Official Synonym Full Names
acyl-CoA synthetase long chain family member 6
NCBI Official Symbol
ACSL6
NCBI Official Synonym Symbols
ACS2; FACL6; LACS2; LACS5; LACS 6
UniProt Protein Name
Long-chain-fatty-acid--CoA ligase 6
UniProt Synonym Protein Names
Long-chain acyl-CoA synthetase 6; LACS 6
Protein Family
Long-chain-fatty-acid--CoA ligase
UniProt Gene Name
ACSL6
UniProt Synonym Gene Names
ACS2; FACL6; KIAA0837; LACS5; LACS 6
NCBI Protein Information
long-chain-fatty-acid--CoA ligase 6
NCBI Summary for ACSL6
The protein encoded by this gene catalyzes the formation of acyl-CoA from fatty acids, ATP, and CoA, using magnesium as a cofactor. The encoded protein plays a major role in fatty acid metabolism in the brain. Translocations with the ETV6 gene are causes of myelodysplastic syndrome with basophilia, acute myelogenous leukemia with eosinophilia, and acute eosinophilic leukemia. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2011]
Show more UniProt Comments for ACSL6
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-ACSL6 antibody
| Reference Product | PubMed Publications |
|---|---|
| PHYH antibody | >1 publications with ACSL6 and PHYH |
Pathways associated with anti-ACSL6 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Adipocytokine Signaling Pathway antibodies | Adipocytokine Signaling Pathway Diagram |
| Adipocytokine Signaling Pathway antibodies | Adipocytokine Signaling Pathway Diagram |
| Fatty Acid Beta Oxidation Pathway antibodies | Fatty Acid Beta Oxidation Pathway Diagram |
| Fatty Acid Biosynthesis Pathway antibodies | Fatty Acid Biosynthesis Pathway Diagram |
| Fatty Acyl-CoA Biosynthesis Pathway antibodies | Fatty Acyl-CoA Biosynthesis Pathway Diagram |
| Fatty Acid Metabolism Pathway antibodies | Fatty Acid Metabolism Pathway Diagram |
| Fatty Acid Metabolism Pathway antibodies | Fatty Acid Metabolism Pathway Diagram |
| Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies | Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram |
| Metabolism Pathway antibodies | Metabolism Pathway Diagram |
| Metabolism Of Lipids And Lipoproteins Pathway antibodies | Metabolism Of Lipids And Lipoproteins Pathway Diagram |
Diseases associated with anti-ACSL6 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Fatty Liver Antibodies | >3 publications with ACSL6 and Fatty Liver |
| Necrosis Antibodies | >2 publications with ACSL6 and Necrosis |
| Eosinophilia Antibodies | >1 publications with ACSL6 and Eosinophilia |
| Seizures Antibodies | >1 publications with ACSL6 and Seizures |
| Liver Diseases Antibodies | >1 publications with ACSL6 and Liver Diseases |
| Nervous System Diseases Antibodies | >1 publications with ACSL6 and Nervous System Diseases |
| Drug Toxicity Antibodies | >1 publications with ACSL6 and Drug Toxicity |
| Inflammation Antibodies | >1 publications with ACSL6 and Inflammation |
Organs/Tissues associated with anti-ACSL6 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >9 publications with ACSL6 and Brain |
| Liver Antibodies | >3 publications with ACSL6 and Liver |
| Testis Antibodies | >3 publications with ACSL6 and Testis |
| Muscle Antibodies | >2 publications with ACSL6 and Muscle |
| Blood Antibodies | >2 publications with ACSL6 and Blood |
| Bone Antibodies | >1 publications with ACSL6 and Bone |