Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

ADHP [119171-73-2]

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Cat# T18912-5mg

Size : 5mg

Marca : TargetMol

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ADHP

(Synonyms: 10-Acetyl-3,7-dihydroxyphenoxazine) Copy Product Info

ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

Cas No. 119171-73-2

For research use only—not for human use. No sales to individuals. Use as intended only.

Batch Information

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Purity:99.57%

Appearance:Solid

Color:White to Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).
In vitro	To obtain the parameters Km and kcat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6/s. The kobs also increase over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second-order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s.
Synonyms	10-Acetyl-3,7-dihydroxyphenoxazine

Chemical Properties

Molecular Weight	257.241
Formula	C₁₄H₁₁NO₄
Cas No.	119171-73-2
Smiles	CC(N1C2=C(C=C(O)C=C2)OC3=CC(O)=CC=C13)=O
Relative Density.	1.459g/cm3

Storage & Solubility Information

Storage

Store at low temperature,Store under nitrogen,Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60.00 mg/mL (233.24 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8874 mL	19.4370 mL	38.8741 mL	194.3703 mL
5 mM	0.7775 mL	3.8874 mL	7.7748 mL	38.8741 mL
10 mM	0.3887 mL	1.9437 mL	3.8874 mL	19.4370 mL
20 mM	0.1944 mL	0.9719 mL	1.9437 mL	9.7185 mL
50 mM	0.0777 mL	0.3887 mL	0.7775 mL	3.8874 mL
100 mM	0.0389 mL	0.1944 mL	0.3887 mL	1.9437 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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