Agaricus Bisporus Lectin (ABA)
Cat# L1222-10
Size : 10mg
Marca : GLYcoDiag
Agaricus bisporus agglutinin (ABA)
| Reference | Short Name | Common Name | Inhibitory Carbohydrate | Glycans Structures Specifities |
|---|---|---|---|---|
| L1222 | ABA | Agaricus Bisporus | Gal/GalNAc | Gal(β-1,3) GaINAc |
Agaricus bisporus agglutinin (ABA) is isolated from button mushrooms and purified by affinity column chromatography on a Bovine Submaxillary Mucin (BSM)- Sepharose 4B gel. This tetrameric lectin is composed of four 16 kDa subunits (1).
ABA is a glycoprotein containing fucose, mannose, galactose and glucosamine glycans residues (1).
Moreover, ABA shows affinity towards (2):
- Gal-exposed O-linked glycans ;
- Galactose (β1,3) N-acetylgalactosamine ;
- GlcNAc-exposed N-linked glycans.
ABA agglutinates animal erythrocytes (3) and is known to detect Antigen T (2). It could also be used in order to isolate subclasses of human IgA (4). This lectin could be a potential therapeutic tool for inflammatory autoimmune pathologies (5) or even cancer (6,7).
Applications
Validated in GLYcoPROFILE®
O-glycans biomarker detection
Agglutination studies
Detection of Antigen T
Isolation of IgA subclasses
Study of inflammatory autoimmune pathologies
Cancer research
Features
Gal and GalNAc binding lectin
Ordering informations
| Reference | Name | Quantity |
|---|---|---|
| L1222-2 | ABA lectin | 2 mg |
| L1222-10 | ABA lectin | 10 mg |
References
(1) Sueyoshi et al., (1985) Purification and characterization of four isolectins of mushroom (Agaricus bisporus). Biol. Chem., 366, 213-221.
(2) Nakamura-Tsuruta et al. (2006) Evidence that Agaricus bisporus agglutinin (ABA) has dual sugar-binding specificity. Biochem. Biophys. Res. Commun., 347, 215-220.
(3) Presant et al., (1972) Characterization of the cell surface receptor for the Agaricus bisporus Hemagglutinin. J. Biol. Chem., 247-21, 6937-6945.
(4) Irazoqui et al. (1992) Differential reactivity of Agaricus bisporus lectin with human IgA subclasses in gel precipitation. J.Immunol. Methods, 156, 199-204.
(5) Ditamo et al (2016) In vivo immunomodulatory effect of the lectin from edible mushroom Agaricus bisporus. Food Funct., 7,262-269.
(6) Wu et al., (2003) Effect of polyvalencies of glycotopes on the binding of a lectin from the edible mushroom Agaricus bisporus. J. Biochem., 371, 311–320.
(7) Yu and al., (1993) Reversible inhibition of proliferation of epithelial cell lines by Agaricus bisporus (edible mushroom)lectin. Cancer Res., 53, 4627-4632.
| Reference | Short Name | Common Name | Inhibitory Carbohydrate | Glycans Structures Specifities | |
|---|---|---|---|---|---|
| L1222 | ABA | Agaricus Bisporus | Gal/GalNAc | Gal(β-1,3) GaINAc | |
| L1221 | AIA / Jacalin | Artocarpus intergrifolia | Gal | Galα1-6 or Galβ1-3GalNAc (T-antigen)>> lactose, more specific for T-antigen than PNA | |
| L1367 | AML | Astragalus membranaceus | Gal | Galβ | |
| L1205 | ASA | Allium sativum agglutinin | α(1,3)Man | Mannose | |
| L1889 | BanLec | Musa acuminata | Mannose, Glucose | αMan | |
| L1255 | BC2L-A | Burkholderia cenocepacia | α(1,3)Man | Mannose-containing ligands | |
| L1256 | BC2L-C | Burkholderia cenocepacia | Lewis b / Lewis Y | Fucose containing ligands | |
| L1254 | CJA | Crotalaria juncea | lactose | Gal > GalNAc | |
| L1366 | cMol | Moringa oleifera | fetuin, thyroglobuline | GlcNAc, Complex carbohydrate-binding specificity | |
| L1201 | Con A | Concanavalin A | MeαMan | α-Man, Glc | |
| L1249 | CorM | Coregonus lavaretus marenae | Rhamnose | Rhamnose |
|