Affinity-purified Amaranthus caudatus (ACL) (SKU: 21510158) is a dimer glycoprotein made up of two subunits. ACL has preferential specificity to oliogsacharrides containing the galactosyl disacharride Galβ3GalNAc. ACL tolerates sialic acid substitution at the 3 positions of galactose in the 'T' antigen. It does not have specificity for blood groups but does have a higher affinity for type O erythrocytes over B and A.

ACL is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers. This product comes in a stabilized liquid form.

Recommended Usage: Recommended dilutions from 0.5 – 10 µg / ml in 1XPBS. Less than 1 µg/ml of ACL will agglutinate human, sheep, goat, rat, rabbit and guinea-pig erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Cell sorting, Glycobiology.

References

1. Rinderle SJ, Goldstein IJ, Matta KL, Ratcliffe RM. Isolation and characterization of amaranthin, a lectin present in the seeds of Amaranthus caudatus, that recognizes the T- (or cryptic T)-antigen. J Biol Chem. 1989 Sep
25;264(27):16123-31.

2. Rinderle SJ, Goldstein IJ, Remsen EE. Physicochemical properties of amaranthin, the lectin from Amaranthus caudatus seeds. Biochemistry. 1990 Nov 20;29(46):10555-61.