Mouse monoclonal antibody Pacific Blue labled to CD8a

Cat# CFP8573-100

Size : 100Assays

Marca : Cohesion Biosciences

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Product NameAnti-CD8a Antibody-Pacific Blue labled
Cat No: CFP8573
Source: Mouse
Reactivity: H
Applications: IF, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Description: Mouse monoclonal antibody Pacific Blue labled to CD8a
Purification: The antibody was purified by affinity chromatography.
Clonality: Monoclonal (clone: HIT8a)
Conjugation: Pacific Blue
Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Dilution: 10 μl / assay
Gene Symbol: CD8A
Alternative Names: MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a
Entrez Gene (Human):
SwissProt (Human):
Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.