Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Anti-Collagen 1, alpha 1 telopeptide Antibody

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Cat# 322-COLT

Size : 100uL

Marca : Phosphosolutions

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Anti-Collagen 1, alpha 1 telopeptide Antibody

Our Anti-Collagen 1, alpha 1 telopeptide primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, rat, and sheep Collagen 1, alpha 1 telopeptide and is antigen affinity purified. It is great for use in WB, IHC.

Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (Cat. 322-COLT, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.

Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (Cat. : 322-COLT, red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.

Western blot of rat lung lysate showing specific immunolabeling of the ~140 kDa collagen protein.

Immunolabeling of a hiPSCs seeded sponge section cultured for 28 days labeling COL1A1 (Cat. 322-COLT, 1:100, green) expression and nuclei are labeled with DAPI. Image from publication, CC-BY-4.0. PMID: 39664802.

Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (Cat. 322-COLT, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.

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Species Reactivity

Human, Mouse, Rat, Sheep

Applications

IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

SKU: 322-COLT

Ships: 1-2 business days

Product Details

Target

Collagen 1, alpha 1 telopeptide

Scientific Background

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the most abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the first 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

COL1A1

Molecular Weight

140 kDa

Immunogen

Synthetic peptide corresponding to amino acid residues specific to the C-terminal region of human collagen I alpha 1 telopeptide, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat, Sheep

Expected Reactivity

Bovine, Canine, Chicken, Feline, Finch, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Vole, Xenopus

Antibody Registry ID

AB_2492061

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Affinity purified rabbit serum.

Buffer

PBS

Dilution Ranges

WB: 1:1000
IHC: 1:100-1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~140 kDa collagen I alpha-1 telopeptide portion of the collagen I alpha-1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0) / 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Alpha 1 type I collagen antibody, Alpha 2 type I collagen antibody, alpha 2 type I procollagen antibody, alpha 2(I) procollagen antibody, alpha 2(I)-collagen antibody, Alpha-1 type I collagen antibody, alpha1(I) procollagen antibody, CO1A1_HUMAN antibody, COL1A1 antibody, COL1A2 antibody, collagen alpha 1 chain type I antibody, Collagen alpha-1(I) chain antibody, collagen alpha-1(I) chain preproprotein antibody, Collagen I alpha 1 polypeptide antibody, Collagen I alpha 2 polypeptide antibody, collagen of skin tendon and bone, alpha-1 chain antibody, collagen of skin tendon and bone alpha-2 chain antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, EDSC antibody, OI1 antibody, OI2 antibody, OI3 antibody, OI4 antibody, pro-alpha-1 collagen type 1 antibody, type I proalpha 1 antibody, type I procollagen alpha 1 chain antibody, Type I procollagen antibody

UniProt ID

P02452

NCBI Gene ID

1277

Shipping

Blue Ice

Western Blot Protocol: Download

Anti-Collagen 1, alpha 1 telopeptide Antibody

Cat# 322-COLT

Marca : Phosphosolutions

Anti-Collagen 1, alpha 1 telopeptide Antibody

SKU: 322-COLT

Product Details

Bulk Order Anti-Collagen 1, alpha 1 telopeptide Antibody